This book describes state-of-the-art approaches for identification and functional characterization of plant peptide hormones and growth factors. Chapters guide readers through protocols for computational identification of novel signaling peptides and phytocytokines, analysis of post-translational maturation events, isolation of mature peptides from plant tissues, peptide bioassays, in vivo and in vitro analysis of peptide activity, peptide receptor identification and interaction analysis, and quantitative assessment of peptide ligand binding to cognate receptors. Written in the highly...

This highly anticipated second edition details cutting-edge experimental approaches for bacteriophage research. This comprehensive volume details new and updated experimental approaches for isolating, formulating, and engineering bacteriophage medicinal products. Authored by leading experts and written in the highly successful Methods in Molecular Biology series format, each chapter includes introductions to their respective topics, lists of the necessary materials and reagents, step-by-step reproducible laboratory protocols, and valuable tips for troubleshooting and avoiding...

This volume explores the latest approaches used to assess trophoblast angiogenesis, transport function, cellular respirations, migration, and invasion. The chapters in this book cover topics such as various methods to study and manipulate primary trophoblast cells; strategies using liposomes to deliver biomolecules to trophoblasts; in vitro models of the placenta that emulate the cellular interactions in the 3D uterine environment; and models exploring heterogenous cell types and 3D-organoid structures that represent cell-to-cell interactions in vivo. Written in the highly successful...

This volume details multiple areas of new and emerging methods to develop the next generation of immunotherapy treatments. Chapters guide readers through analysis and characterisation of the interactions between tumour and immune cells, and cell engineering tools for cancer treatment, to provide a unique and compelling set of techniques instrumental to work with, and engineer, immune cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents,...

This volume will serve as a guide for students in the field of neurobiology, and be a bridge between basic science researchers, doctors, and surgeons in clinical practice. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Neurobiology: Methods and Protocols aims to ensure successful results in...