This detailed volume explores methods currently used to investigate the cell wall of various bacterial species and pathogens. By using a combination of genetic, molecular, biochemical, and cytological techniques, the protocols address many fundamental questions involving the composition, biosynthesis, and regulation of bacterial peptidoglycan. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well...
This detailed volume explores methods currently used to investigate the cell wall of various bacterial species and pathogens. By using a combination o...
This third edition volume expands on the previous editions with new discussions on the latest techniques and developments in the field. The chapters in this book are organized into four parts, and cover topics such as optical tweezers; single-molecule fluorescence tools; atomic force microscopy; magnetic tweezers; applications to virus protein shells, unfolding of proteins, nucleic acids, motor proteins, in vivo and in vitro; and protocols to establish specific surface interactions and perform force calibration. Written in the highly successful Methods in Molecular Biology series...
This third edition volume expands on the previous editions with new discussions on the latest techniques and developments in the field. The chapters i...
This volume details the development of updated dry lab and wet lab based methods for the reconstruction of Gene regulatory networks (GRN). Chapters guide readers through culprit genes, in-silico drug discovery techniques, genome-wide ChIP-X data, high-Throughput Transcriptomic Data Exome Sequencing, Next-Generation Sequencing, Fuorescence Spectroscopy, data analysis in Bioinformatics, Computational Biology, and S-system based modeling of GRN. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective...
This volume details the development of updated dry lab and wet lab based methods for the reconstruction of Gene regulatory networks (GRN). Chapters...
This detailed volume explores a variety of techniques used to study lineage commitment in stem cells. Further elucidation of the process that stem cells undergo on their way to becoming more specified cell types is vital for a more complete understanding of cell biology and overall physiology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known...
This detailed volume explores a variety of techniques used to study lineage commitment in stem cells. Further elucidation of the process that stem cel...
This second edition provides detailed techniques used for the study and characterization of the plant vascular system, with a central focus on the xylem tissue. Chapters are organized in three main sections covering; analysis of xylem development, xylem characterization though imaging techniques, and analysis of the xylem composition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key...
This second edition provides detailed techniques used for the study and characterization of the plant vascular system, with a central focus on the ...
This volume covers the latest advancements in the study of ciliary complexity. Protocols cover genomic, proteomic, imaging, and functional analysis of different ciliated tissues and their wide applicability in cilia biology. Chapters in this book primarily focus on methods to study multiciliated cells, and discuss topics such as SARS-CoV-2 infections of human primary nasal multiciliated epithelial cells; expansion microscopy of ciliary proteins; live-imaging centriole amplification in mouse brain multiciliated cells; biophysical properties of cilia motility; and mucociliary transport...
This volume covers the latest advancements in the study of ciliary complexity. Protocols cover genomic, proteomic, imaging, and functional analysis...
This second edition details new and updated protocols that cover techniques used to study secretion systems. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as using reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters...
This second edition details new and updated protocols that cover techniques used to study secretion systems. Chapters focus on identifying and loca...
This book provides insight into the biology of Wolbachia with a particular focus on methodology. Chapters guide readers through reproductive phenotypes caused by Wolbachia, survival and replication of Wolbachia in insect cell lines, artificial introduction of Wolbachia into novel hosts, and analysis of Wolbachia gene functions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily...
This book provides insight into the biology of Wolbachia with a particular focus on methodology. Chapters guide readers through reproduct...
This detailed volume explores key concepts and experimental design related to Positron Emission Tomography (PET) imaging that have revolutionized our understanding of human biology. The first part focuses on recent advances in radiotracer probe development to enable the detection of materials, from large macromolecules to complicated drug-like structures. The next section describes how key physiological and pathophysiological processes can be interrogated and quantifiably measured with this imaging technique. Finally, chapters examine important technological developments in the field that are...
This detailed volume explores key concepts and experimental design related to Positron Emission Tomography (PET) imaging that have revolutionized our ...
This second edition volume expands on the previous edition with discussions of the latest advancements and methods used by scientists to study systems biology. The chapters in this book are organized into four parts. Part One looks at models in systems biology and parameters identification such as short peptide analysis, metastasis models, and metabolomics. Part Two covers computational methods in the study of organisms, and cancer non-linear dynamics. Part Three discusses critical transition states across Waddington's like landscapes such as understanding cell differentiation through...
This second edition volume expands on the previous edition with discussions of the latest advancements and methods used by scientists to study systems...
