The second volume of the series Manuals in Biomedical Research, this book is aimed to be both a concise introduction to the diverse field of microscopy and a practical guide to those who require the use of microscopy methods in their research. It provides young as well as experienced scientists a state-of-the-art multidisciplinary overview of microscopy techniques, covering all the major microscopy fields in biomedical sciences and showing their application in evaluating samples ranging from molecules to cells and tissues. Microscopy has revolutionized our understanding of biological events....

Biochemistry plays an important role in all areas of the biological and medical sciences. With most of the research or diagnosis involved in these areas being based on biochemically obtained observations, it is essential to have a profile of well standardized protocols. This manual is a basic guide for all students, researchers and experts in biochemistry, designed to help readers in directly starting off their experiments without prior knowledge of the protocol. The book dwells on the concepts used in designing the methodologies, thereby giving ample room for researchers to modify them...

Tissue engineering has been recognized as offering an alternative technique to whole-organ and tissue transplantation for diseased, failed, or malfunctioned organs. To reconstruct a new tissue via tissue engineering, the following triad components are needed: (1) cells which are harvested and dissociated from the donor tissue; (2) biomaterials as scaffold substrates in which cells are attached and cultured, resulting in implantation at the desired site of the functioning tissue; and (3) growth factors which promote and/or prevent cell adhesion, proliferation, migration, and differentiation....

Laboratory animal research remains a very important part of basic research and drug development. With the worldwide increase in biotechnology, more and more researchers are required to use animals for research. However, many have basic or little training in experimental techniques or in the background information, which remains very important. This book rectifies the problem by providing animal researchers and technicians with the essentials for conducting their work in the laboratory, offering detailed protocols and information that can be referred to on a daily basis. Broadly covering a...

This manual is designed to serve as a practical guide to primary human cell culture, which is integral in both academic and industrial biotechnology research. As in the first edition, the content of the manual is not exhaustive, but rather contains selected protocols for specific cell types from major tissue groupings in the body. This improved second edition also includes a new section on stem cells and additional material on transfection. It should serve as a foundation for individual researchers to experiment, explore, and establish niche protocols for their specific needs. With its...

This is the first experimental protocol book that covers the differentiation of bone marrow derived stem cell (BMSC) into specific cell type, targeted at the undergraduate and graduate student level. The 19 chapters deal with the differentiation methods using small molecules, cytokines and polymeric scaffolds. BMSCs are pluripotential in that they not only act as myelo-regenerative and supportive cells, but can also differentiate into almost kinds of cells in our body. In addition, when implanted in vivo, they could help repair multiple tissue such as blood vessels, heart, liver and so on. ...