As part of the boom in biotechnology, particularly in tissue engineering, primary human cell culture has become a major pillar in academic research and the biopharmaceutical industry. Obtaining a viable culture from a tissue sample and maintaining it for experimental, diagnostic or therapeutic purposes can be quite a challenge. Based on laboratory protocols and practical experience from many years of primary cell culture, this manual presents the basic steps necessary for culturing primary human cells. Written by students for students, the manual serves well as a practical guide to primary...

This manual is designed to serve as a practical guide to primary human cell culture, which is integral in both academic and industrial biotechnology research. As in the first edition, the content of the manual is not exhaustive, but rather contains selected protocols for specific cell types from major tissue groupings in the body. This improved second edition also includes a new section on stem cells and additional material on transfection. It should serve as a foundation for individual researchers to experiment, explore, and establish niche protocols for their specific needs. With its...

This is the first experimental protocol book that covers the differentiation of bone marrow derived stem cell (BMSC) into specific cell type, targeted at the undergraduate and graduate student level. The 19 chapters deal with the differentiation methods using small molecules, cytokines and polymeric scaffolds. BMSCs are pluripotential in that they not only act as myelo-regenerative and supportive cells, but can also differentiate into almost kinds of cells in our body. In addition, when implanted in vivo, they could help repair multiple tissue such as blood vessels, heart, liver and so on. ...