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The performance of a passive Q-switching of optical fiber laser system has been studied theoretically. The system consist of Er+3 doped optic fiber as active medium and Cr+4: YAG crystal as a saturable absorber material. The fundamentals of optical fibers and their classifications are explained, as well as the activity of saturable absorber materials, their optical performance, and the properties of passive Q-switched laser pulses investigated.

Micro technique is the preparation and staining of tissue sections from surgical or autopsy material for microscopic studies.Stained tissue is examined by the pathologist who either confirms or makes the final diagnosis of the patients disease and determines or affect the mode of therapy.Cytology is the study of individual cells microscopically.Histology is the study of specialized cell aggregates from definite structures with specific functions.Histotechnologists are the persons who do the tissue processing and make the glass microscopic slides.