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This volume provides readers with a collection of the latest protocols used to study plant genome editing and trait engineering. The chapters in this book are organized into five parts: genome engineering systems; machinery design and validation; delivery tools; generation and analysis of engineering materials; and crop genome engineering applications. The chapters cover topics such as methods of applying the popular CRISPR-Cas9 or CRISPR-Cas12 systems for editing genomes in different crop species, the use of small synthetic plastome for potato genome engineering, and the use of CRISPR-Cas9...

This book constitutes the refereed proceedings of the 5th EAI International Conference on Smart Grid and Internet of Things, SGIoT 2021, held in TaiChung, Taiwan, in December 2021.The 9 regular papers and 4 short papers presented were carefully reviewed and selected from 57 submissions. The papers cover a broad range of topics in wireless sensor, vehicular ad hoc networks, security, deep learning and big data. The papers are organized in two subject areas: applications on internet of things, and communication security, big data, neural networks and machine learning.