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Lectins: Methods and Protocols summarizes classic lectin technologies and advanced techniques with high throughputs and sensitivities. Chapters include methods and techniques for serial lectin-affinity chromatography procedure, lectin-probed western blot and histochemical analyses, quantitative interaction analyses based on equilibrium dialysis, isothermal calorimetry, surface plasmon resonance, techniques for elucidating functions of endogenous animal lectins, advanced methods on "engineer" novel lectins by evolutionary concepts, and special equipment. Written in the highly...

Lectins: Methods and Protocols summarizes classic lectin technologies and advanced techniques with high throughputs and sensitivities. Chapters include methods and techniques for serial lectin-affinity chromatography procedure, lectin-probed western blot and histochemical analyses, quantitative interaction analyses based on equilibrium dialysis, isothermal calorimetry, surface plasmon resonance, techniques for elucidating functions of endogenous animal lectins, advanced methods on engineer novel lectins by evolutionary concepts, and special equipment. Written in the highly...

In 1943, University of Washington student Gordon Hirabayashi defied the curfew and mass removal of Japanese Americans on the West Coast, and was subsequently convicted and imprisoned as a result. In A Principled Stand, Gordon's brother James and nephew Lane have brought together his prison diaries and voluminous wartime correspondence to tell the story of Hirabayashi v. United States, the Supreme Court case that in 1943 upheld and on appeal in 1987 vacated his conviction. For the first time, the events of the case are told in Gordon's own words. The result is a compelling and...

In 1943, University of Washington student Gordon Hirabayashi defied the curfew and mass removal of Japanese Americans on the West Coast, and was subsequently convicted and imprisoned as a result. In A Principled Stand, Gordon's brother James and nephew Lane have brought together his prison diaries and voluminous wartime correspondence to tell the story of Hirabayashi v. United States, the Supreme Court case that in 1943 upheld and on appeal in 1987 vacated his conviction. For the first time, the events of the case are told in Gordon's own words. The result is a compelling and...

Sphingolipids are fundamental to the structures of cell membranes, lipoproteins, and the stratum cornea of the skin. Many complex sphingolipids as well as simpler sphingoid bases and derivatives are highly bioactive as extra- and intracellular regulators of growth, differentiation, migration, survival, senescence, and numerous cellular responses to stress. This book provides many examples of exciting new developments in sphingolipid biology/sphingolipidology that are changing our understanding of how multicellular organisms grow, develop, function, age, and die. Key areas addressed include...