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Plant phenolics represent a huge number of natural compounds that are of great interest and importance nowadays due to their various pharmacological activities. The aqueous methanolic extract of Acacia Saligna was applied on Polyamide 6S and eluted with water and water/ethanol mixtures with gradual increase in concentration. The successive extraction of plant afforded the isolation of sixteen phenolic compounds, among them; Catechin, 7-O-Galloyl-Catechin, Myricetin-3-O-α-L-arabinopyranoside, Quercetin-3-O-β-D-glucopyranoside, Quercetin-3-O-α-L-arabinopyranoside,...