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Tuberculosis is devastating disease at global level. TB is caused by members of Mycobaterium tuberculosis complex group. Presently available biochemical methods of diagnosis of tuberculosis are not accurate. The highly sensitive polymerase chain reaction (PCR) and specific molecular probes techniques are major advances in early diagnosis and molecular epidemiology of various diseases including TB. It is established that the ribosomal gene region of both the prokaryotic and eukaryotic comprise of sequences that are conserved during evolution interspersed with sequence which are divergent. The...

Tuberculosis caused by Mycobacterium tuberculosis is rampant in human and animals; it has been classified as reemerging disease as it is being reported with increasing frequency in human particularly from developing countries. Early diagnosis of the disease by using sensitive, specific, rapid & cost effective diagnostic procedure is of utmost importance for effective control. Although several modern methods like BACTEC, ATP Bioluminescent, Biological Oxygen uptake assay, MGIT, r-RNA viability etc. are available for clinical use. These techniques are rapid, radioactive (BACTEC), sensitive,...