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Gene pyramiding approach was used to
produce transgenic cotton expressing two Bt genes for
sustainable resistance. The vector construct pK2Ac
contained Bt genes and nptII gene both driven by CaMV35S
promoter, confers resistance to
Kanamycin.Sonication-assisted Agrobacterium-mediated
transformation system was used. Heliothis armigera 2nd
instar larval mortality ranged 75-100% and Bt protein was
quantified in the leaves of transgenic plants. Segregation
showed inheritance pattern 3:1...

My research covers the area of interaction of light in vital functioning of plants. Yield and development of specific trait is the ultimate goal of my research especially in area of gene transformation. There are two ways to achieve yield goal via transformation of insect resistant gene like Cry1Ac or to increase the physiological efficiency of plants via genetical modification. The latter methodology was adopted as done by Thiele et al in potato. Here, the same methodology was adopted in improving the physiology of cotton plants by transforming it with Phy B gene to enhance photosynthetic...

Cotton being a cash crop contributes significantly to foreign exchange earnings.Presently cotton crop is threatened by the attack of insects and viruses like whitely and cotton leaf curl virus (CLCuV). To overcome this problem different genetic engineering techniques are being adopted. Among them RNA interference based resistance has proven to be reliable approach. RNA interference is a complex and conserved mechanism utilized by plants and other eukaryotes to protect themselves. The use of RNA interference provides environmental friendly approach to generate plants resistant to insects and...