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A biosensor is an integrated bioanalytical device that employs biological elements, as analyte sensor, coupled to a transducer for signal detection. Biosensor provides sensitive and selective determination of various compounds of analytical relevance. Recently, use of self-assembled monolayer (SAM) is gaining attention as biosensor matrix. SAM provides a simple route to functionalize electrode surfaces for biosensor fabrication. The alarming rise in the rates of heart diseases due to abnormal levels of cholesterol in blood has stimulated public concern about the estimation of cholesterol...