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This handy reference describes the fundamental principles and procedures underlying the successful isolation of viable, functionally intact cells from mammalian endocrine tissues, and their maintenance as primary cultures. The cell types selected for coverage illustrate the diversity of endocrine tissues from which cells have been isolated, and the range of procedures that have been devised to ensure the optimal survival and behavior of each cell type under study. The book places particular emphasis on the provision of detailed protocols describing, step by step, the manipulations necessary...

This handy reference describes the fundamental principles and procedures underlying the successful isolation of viable, functionally intact cells from mammalian endocrine tissues, and their maintenance as primary cultures. The cell types selected for coverage illustrate the diversity of endocrine tissues from which cells have been isolated, and the range of procedures that have been devised to ensure the optimal survival and behavior of each cell type under study. The book places particular emphasis on the provision of detailed protocols describing, step by step, the manipulations necessary...