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Plant tissue culture is recognized worldwide as a creative, promising and feasible option for propagation and genetic manipulation of plant, particularly those with special demands. The most popular application of tissue culture method is in the genetic transformation system. As a tool of genetic engineering of plant, plant regeneration using tissue culture system is taken a crucial part. Another important use of tissue culture system is proliferation of plant, which is an alternative method to vegetative plant multiplication. While conventional propagation methods are used, one cutting...