Chemical Synthesis of Posttranslationally Modified Proteins : From Chemistry to Biology

ISBN-13: 9783659247521 / Angielski / Miękka / 2012 / 156 str.

Renliang Yang

Opis

Many proteins undergo extensive posttranslational modifications (PTMs), such as methylation, acetylation, phosphorylation, glycosylation and ubiquitination. To elucidate the roles of these PTMs, it is necessary to generate homogeneously modified proteins. In this book, we are going to introduce the recently developed chemical synthetic methods for the synthesis of proteins with certain PTM(s). First, we introduce a ligation/S-alkylation approach for the semisynthesis N-terminal tail acetylated proteins. Next, we also introduce the dual native chemical ligation at lysine method for the synthesis of ubiquitinated peptides and proteins. Finally, we introduce a novel N-to-C sequential ligation method for protein total synthesis. These methods developed have dramatically promoted protein PTM research.

O autorze

Yang, RenliangI completed my undergraduate studies in Biological Sciences at Nanyang Technological University with honors in 2007, and received my PhD in Chemical Biology and Biochemistry at the same university in 2012. During my PhD studies, I followed Dr Liu Chuan-Fa and focused on the study of chemical ligation and its application in protein synthesis.