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Biofilms are the 'aggregates of microorganisms in which cells are frequently embedded in a self produced matrix of extracellular polymeric substances (EPS) that are adherent to each other and/or a surface. This text book comprises of basic ultra structure of biofilms, it's development, type, composition , characteristic feature of biofilm and resistance of microbes in biofilms; approaches to biofilm prevention and current therapeutic options for endodontic biofilms explained in simplified manner after reviewing the literature.