Plant and Food Carotenoids: Methods and Protocols

ISBN-13: 9781493999545 / Angielski / Miękka / 2020 / 421 str.

Manuel Rodríguez-Concepción; Ralf Welsch

Spis treści

Part I: Introduction

1. Pathways for Carotenoid Biosynthesis, Degradation, and Storage

Tianhu Sun, Yaakov Tadmor, and Li Li

 

Part II: Activities

2. A Simple in vitro Assay to Measure the Activity of Geranylgeranyl Diphosphate Synthase and Other Short-chain Prenyltransferases

M. Victoria Barja and Manuel Rodriguez-Concepcion

 

3. Expression, Purification, and Enzyme Activity Assay of Phytoene Synthase in vitro

Maurizio Camagna and Ralf Welsch

 

4. Improved Expression and Purification of the Carotenoid Biosynthetic Enzyme Z-ISO

Eleanore T. Wurtzel and Jesús Beltrán

 

5. Determination of In Vitro and In Vivo Activities of Plant Carotenoid Cleavage Oxygenases

Lourdes Gómez-Gómez, Gianfranco Diretto, Oussama Ahrazem, Salim Al-Babili

 

6. Expression and Characterization of Mammalian Carotenoid Cleavage Dioxygenases

Linda Thomas, Srinivasagan Ramkumar, and Johannes von Lintig

 

7. Transportomics for the Characterization of Plant Apocarotenoid Transmembrane Transporters

Olivia Costantina Demurtas, Rita de Brito Francisco, Enrico Martinoia, and Giovanni Giuliano

 

Part III: Analytics

8. Applications of Visible Spectroscopy and Colour Measurements in the Assessments of Carotenoid Levels in Foods

Paula Mapelli-Brahm, Francisco J. Rodríguez-Pulido, Carla M. Stinco; Francisco J. Heredia, and Antonio J. Meléndez-Martínez

 

9. A Routine Method for the Extraction and HPLC-DAD Profiling of Major Plant and Food Carotenoids

Dámaso Hornero-Méndez

 

10. Acquisition of Mass Spectrometry Data of Carotenoids: A Focus on Big Data Management

Antonio Pérez-Gálvez, Isabel Viera, and María Roca

 

11. cis/trans Carotenoid Extraction, Purification, Detection, Quantification, and Profiling in Plant Tissues

Yagiz Alagoz, Namraj Dhami, Chris Mitchell, and Christopher I Cazzonelli

 

12. Determination of Plant Volatile Apocarotenoids

José L. Rambla and Antonio Granell

 

13. A Method for Extraction and LC-MS Based Identification of Carotenoid-Derived Dialdehydes in Plants

Jianing Mi, Kun-Peng Jia, Aparna Balakrishna, and Salim Al-Babili

 

14. Fractionation of Tomato Fruit Chromoplasts

Karel De Pourcq and Albert Boronat

 

15. Quantification of Strigolactones

Carlos Rial, Rosa M. Varela, José M. G. Molinillo, Alexandra G. Durán and Francisco A. Macías

 

16. Carotenoid and Apocarotenoid Analysis by SFE-SFC-QqQ/MS

Daniele Giuffrida, Mariosimone Zoccali, and Luigi Mondello

 

Part IV: Imaging

17. Elucidating Carotenoid Biosynthetic Enzyme Localization and Interactions Using Fluorescent Microscopy

Maria Shumskaya, Rena F. Quinlan, and Eleanore T. Wurtzel

 

18. Visualization of Carotenoid-storage Structures in Fruits by Transmission Electron Microscopy

Joanna Lado, Jaime Zacarias, María Jesús Rodrigo, and Lorenzo Zacarias

 

19. Light Microscopy and Raman Imaging of Carotenoids in Plant Cells in situ and in Released Carotene Crystals

Tomasz Oleszkiewicz, Marta Z. Pacia, Ewa Grzebelus, and Rafal Baranski

 

Part V: Dynamics

20. Carotenoid Isotopologue Profiling in ¹³C-labeled Leaf Extracts by LC-MS and LC-FTICR-MS

Björn Thiele and Shizue Matsubara

 

21. Quantification of Carotenoid Pathway Flux in Green and Non-green Systems

Julian Koschmieder and Ralf Welsch

 

22. Histochemical and Molecular Quantification of Arbuscular Mycorrhiza Symbiosis

Juan M. García, María J. Pozo, and Juan A. López-Ráez

 

Part VI: Biotechnology

23. Use of Potyvirus Vectors to Produce Carotenoids in Plants

José-Antonio Daròs

 

24. Agrobacterium tumefaciens Mediated Stable Transformation of Daucus carota

Christian Gonzalez-Calquin and Claudia Stange

 

25. CRISPR-Cas-Mediated Gene Knockout in Tomato

Gwen Swinnen, Thomas Jacobs, Laurens Pauwels, and Alain Goossens

 

26. Modulation of Activity of a Carotenoid Pathway Through the use of the TET-on Regulatory System: Application in the Fungus Fusarium fujikuroi

Julia Marente, Pedro Ortega, Javier Pardo-Medina, Javier Avalos, and M. Carmen Limón

 

Part VII: Nutrition and Health

27. Intervention Studies in Humans

Begoña Olmedilla-Alonso

 

28. In vitro Digestion for Control and Monitoring of Food Effects In Relation To Micellarization Index of Carotenoids

Antonio Pérez-Gálvez and Javier Fontecha

 

29. Evaluation of Carotenoids Protection against Oxidative Stress in the Animal Model Caenorhabditis elegans

Patricia Martorell, Silvia Llopis, José Vicente Gil, Salvador Genovés, Daniel

Ramón, Lorenzo Zacarías, and María Jesús Rodrigo

 

30. Mouse Models to Study Antiobesogenic Effects of Carotenoids

Joan Ribot, Bojan Stojnic, Andreu Palou, and M. Luisa Bonet

Blurb

This volume provides a comprehensive compilation of techniques and protocols used in plant and food carotenoid research. Chapters guide readers through seven major areas on core enzyme activities, analysis of carotenoid profiles, new imaging tools, synthesis and degradation dynamics, biotechnology, nutrition, and health. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Plant and Food Carotenoids: Methods and Protocols aims to be helpful to researchers of other disciplines that are impacted by carotenoids, including photosynthesis, biotechnology, food science, and nutrition.