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Cattle industry is drastically affected by the trematode parasite Fasciola gigantica. This work aims to study the humoral and cellular immune responses to different F. gigantica antigens isolated from adult worm E/S product during the parasite invasion. This research is an attempt to find an effective vaccine against the parasite.Two Antigens were isolated and purified from excretory/secretory products as cysteine protease and fatty acids binding proteins (CP and FABP) of the parasite by immunoaffinity chromatography. From this study it was deduced that F. gigantica CP is a relevant candidate...