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Sugarcane (Saccharum officinarum L.) is one of the leading crops in the world. Molecular characterization and molecular marker assisted selection in sugarcane breeding might be a tool for improvement of this crop can assist in increasing sugar production. Like many other plant species, sugarcane tissues contain high levels of polysaccharides and polyphenolic compounds, which present a major contamination problem during DNA purification. High-concentrations of polysaccharides, which co-purify with DNA in normal phenol-chloroform extractions and polyphenols covalently bind to DNA making it...