A Practical Guide to Protein and Peptide Purification for Microsequencing

ISBN-13: 9780124802827 / Angielski / Miękka / 1993 / 184 str.

Paul T. Matsudaira

Opis

Why a Second Edition?
The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.

• Mass spectrophotometric characterization of proteins and peptides
• N-terminal sequencing of proteins with blocked N termini
• Internal amino acid sequence analysis after protease digestion in-gel and on-blot
• Improved microscale peptide purification methods
• Computer analysis of protein sequences
• New protocols tested and refined through everyday use in authors' laboratories
• Updated reference chapter covering all aspects of protein microsequencing

Recenzje

Reviews of the First Edition:
"Like the evening network television news programs, this book gives a useful summary of the highlights (of protein chemistry) to enable the masses to go forth armed with enough information to survive in the world." --Cell

Spis treści

Strategies for Obtaining Partial Amino Acid Sequence Data from Small Quantities (5nmol) of Pure of Partially Purified Protein. Enzymatic Digestion of Proteins and HPLC Peptide Isolation
Purification of Proteins and Peptides By SDS-PAGE
Internal Amino Acid Sequence Analysis of Proteins After in Situ Protease Digestion on Nitrocellulose
Mass Spectrometric Strategies for the Structural Characterization of Proteins