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Recent years have seen an increasing interest in metabolic engineering of C. glutamicum for production of organic acids. Here, we report a new engineered strain capable of lysine production, which has 10-fold high lysine production capacity than the wild type strain. In this book we provided further insight into AK regulation and function and also provided valuable information for engineering L-lysine producing C. glutamicum strains. The data provided in this book show the importance of the deregulation of aspartate kinase and site directed mutagenesis for overproduction of L-lysine in C....