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The study of in-vitro micropropagation has assumed enormous importance with the tremendous pace of progress in different disciplines of biological sciences. The tissue culture will play an important role in solving the problems of conventional methods for propagation, hybridization, embryo rescue, production of secondary metabolites, production of virus free plants and paternity disputes. This book help the orchid grower to keep themselves abreast of the latest developments along with methods of mass propagation through various explants as well as conservation of endangered and rare orchids....

In present study, callus was cultured on MS-basal medium with various concentrations and combinations of BAP, NAA, 2, 4-D and kinetin. Large callus were observed in MS medium with additives of 0.5mg/L BAP+1.5 mg/L NAA. Similar chemical group was observed in in-vivo and in-vitro material of Withania somnifera during phytochemical screening. Most of the isolated compounds of Withania somnifera showed activity against drug resistance bacteria. Callus of Withania somnifera contain active phytocompound against the growth of Pseudomonas aeruginosa, Proteus vulgaris and Klebsicela pneumonia than...