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Hepatitis B is a serious health threat in different parts of the world. Pakistan is the country with an intermediate prevalence of Hepatitis B (HBV). Only two twin cities were included in the eight month study of patients of Rahim Yar Khan-Sadiqabad and Rawalpindi-Islamabad. In the present study the rate of seroprevalence and risk factors among the patients of Rahim Yar Khan-Sadiqabad and Rawalpindi-Islamabad was determined.

This study was carried out to observe inter-strain variation at molecular level in the two vaccinal types (B: 3, 4 and B: 6). The 85, 27 kDa major and 70 and 40 kDa minor proteins run on SDS-PAGE were common in both strains. The 50, 32 and 29 kDa major proteins were found in B: 3, 4 but absent in B: 6. SDS-PAGE profiles of outer membrane protein (OMP) showed that 26 minor bands were common. The 50 and 32 kDa major protein were found in B:3,4 and absent in B:6, while 46kDa minor protein was found in B:6 only. The spectral peaks of whole cells, envelope and OMPs could be differentiated between...