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In this third edition the authors have written a guide to the essentials of plant tissue culture. The book takes the reader through a graded series of experimental protocols and provides an introductory review of each topic. There are discussions of aseptic techniques, and nutritional components of media. Subsequent chapters cover such topics as callus formation, organogenesis, xylem cell differentiation, root cultures, cell suspensions, micropropagation by buds, somatic embryogenesis, protoplast culture and fusion, secondary metabolite production, and quantitation of procedures. A glossary,...

The main objective of the book is to provide an up-to-date examination of the possible roles of plant hormones during the cytodifferentiation of xylem and phloem elements in higher plants. Various facets of vascular differentiation, as cell determination, cell cycle activity, and the biochemical events in xylogenesis, are analyzed. Furthermore, the latest information on the roles of auxins, cytokinins, gibberellins, ethylene, and abscisic acid during vascular cell formation are summarized. A theoretical discussion of the six-point hypothesis and the vascular adaptation hypothesis is included....