Flavins and Flavoproteins 1993: Proceedings of the Eleventh International Symposium, Nagoya, Japan, July 27-31, 1993

ISBN-13: 9783110141658 / Angielski / Twarda / 1994 / 909 str.

Kunio Yagi

Spis treści

Frontmatter -- PREFACE -- Committees -- CONTENTS -- THEORETICAL AND CHEMICAL APPROACH -- Use of Molecular Orbital Calculations in Studies on Mechanisms of Enzyme Catalysis -- A theoretical approach of the mechanism of C(4a)-peroxyflavin catalyzed reactions -- Semiempirical calculations on properties of (iso)alloxazines in ground and excited states -- Pathways for a 4a-hydroxy-4a,5-dihydroflavin radical in the hydroxylation of aromatics -- CHEMICAL SYNTHESIS OF NEKOFLAVIN -- NOVEL 5 - SUBSTITUTED 5 - DEAZAFLAVINS : SYNTHESIS AND APPLICATIONS AS ACTIVE SITE PROBES FOR FLAVOPROTEINS -- D-Lactate Dehydrogenase Model. Oxidation of a-Hydroxy Acid by Functionalized Oxidation Active flavin Mimic in the Presence of Zn2+ and Base in t-Butanol -- Mechanism of Flavin Reduction By Cu(I)-EDTA Complex -- SELF-ASSOCIATION OF FLAVIN: INTERACTION OF FMN WITH ALBUMIN -- BIOSYNTHESIS OF FLAVINS -- STUDIES ON THE BIOSYNTHESIS OF FLAVINS. STRUCTURE AND MECHANISM OF 6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE -- BIOSYNTHESIS OF RIBOFLAVIN. CLONING, SEQUENCING, MAPPING, AND HYPEREXPRESSION OF THE GENES ribA CODING FOR GTP CYCLOHYDROLASE II AND ribC CODING FOR RIBOFLAVIN SYNTHASE OF ESCHERICHIA COLL -- BIOSYNTHESIS OF RIBOFLAVIN: ENZYMATIC FORMATION OF 6,7-DIMETHYL-8-RIBITYLLUMAZINE IN Saccharomyces cerevisiae -- ELECTRON MICROSCOPIC STUDIES ON THE LUMAZINE SYNTHASE/ RIBOFLAVIN SYNTHASE COMPLEX OF BACILLUS SUBTILIS -- 19F NMR STUDIES ON LUMAZINE PROTEIN FROM PHOTOBACTERIUM PHOSPHOREUM -- 19F NMR STUDIES ON THE MECHANISM OF RIBOFLAVIN SYNTHASE -- OXIDASES -- STUDIES ON ACTIVE SITE MUTANTS OF SPINACH GLYCOLATE OXIDASE -- HIGH-LEVEL EXPRESSION OF RAT KIDNEY HYDROXY ACID OXIDASE IN ESCHERICHIA COLI: PURIFICATION AND CHARACTERIZATION OF THE RECOMBINANT PROTEIN -- The Influence of Substrate Structure on the Redox Reactions of Monoamine Oxidases -- QUANTITATIVE STRUCTURE-ACTIVITY RELATIONSHIPS IN THE OXIDATION OF BENZYLAMINE ANALOGUES BY BOVINE LIVER MONOAMINE OXIDASE B -- Experimental Probes of Hydrogen Tunneling in Bovine Liver Monoamine Oxidase B -- Stereospecificity and Deuterium Isotope Effect in the Oxidative Deamination Catalyzed by Flavine and Non-flavine Amine Oxidases -- CHARACTERIZATION OF L-ASPARTATE OXIDASE OVEREXPRESSED IN E. COLI -- IDENTIFICATION OF ANOTHER SUBUNIT IN L-PHENYLALANINE OXIDASE FROM PSEUDOMONAS SP. P-501 AND THE PRIMARY STRUCTURE OF THE SUBUNIT -- Kinetic Isotope Effects on Reductive Half-Reactions of Flavoprotein Oxidases -- Effects of Ligands on the Reactivities of Reduced and Semiquinoid Forms of D-Amino Acid Oxidase -- Thermodynamic Study of FAD Binding in D-Amino Acid Oxidase -- Structure of Human D- Amino Acid Oxidase Gene -- AMINO ACID SEQUENCE OF D-AMINO ACID OXIDASE FROM THE YEAST Rhodotorula gracilis -- CHEMICAL MODIFICATION OF ARGEVINE GROUPS IN D-AMINO ACID OXIDASE FROM Rhodotorula gracilis Involvement in catalysis and assignment in the sequence -- FUNCTIONAL AND STRUCTURAL ASPECTS OF D-AMINO ACID OXIDASE FROM Rhodotorula gracilis PROBED BY LIMITED PROTEOLYSIS -- THE CRYSTAL STRUCTURE OF CHOLESTEROL OXIDASE. MECHANISTIC IMPLICATIONS FOR FAD DEPENDENT ALCOHOL OXIDATION -- Nitroalkane-Oxidizing Flavoenzymes -- A 340-nm Chromophore of Nitroalkane Oxidase from Fusarium oxysporum -- OXYGENASES -- LACTATE MONOOXYGENASE: STUDIES OF ACTIVE SITE MUTATIONS -- The Mobile Flavin of Parahydroxybenzoate Hydroxylase - A Case for Major Structural Dynamics in Catalysis -- Structures of Mutant p-Hydroxybenzoate Hydroxylases: Evidence for an Alternative Mode of Flavin Binding -- Substrate and Effector Specificity of Two Active-Site Mutants of p-Hydroxybenzoate Hydroxylase from Pseudomonas fluorescens -- Solvent Isotope Effects on p-Hydroxybenzoate Hydroxylase -- IMMUNOLOGICAL STUDIES ON THE STRUCTURE OF 4-AMINOBENZOATE HYDROXYLASE FROM AGARICUS BISPORUS -- STUDIES ON p-HYDROXYPHENYLACETATE-3-HYDROXYLASE -- Fluorescent Intermediates and the Reaction Mechanism of Phenol Hydroxylase -- Application of pulse radiolysis to produce absorbing spec