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The book explores the instrumentation and applications of optical tweezers to study statistical physics in soft, bio and nanoscale systems. The first part gives a general introduction about optical tweezers and detailed description of a typical setup along with its calibrations. Then it describes studies of statistical properties in soft matter systems, namely, out-of-equilibrium microrheology in a worm-like micellar system, irreversibility to reversibility crossover in the non-equilibrium trajectories of an optically trapped bead, and tunable Brownian vortices at liquid-air interface. The...