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SOME ADVANTAGES OF CELL-BASED BIOSENSORS:
Isolation, purification, and immobilization of enzymes are often very difficult; these can be omitted with cell-based biosensors.
Some enzymes can lose their activity during isolation or immobilization; this risk is eliminated by the use of whole cells.
Enzymes in the cell's natural environment are usually extremely stable.
Multi-step enzyme reactions in intact cells can be used, making it possible to avoid the preparation of complicated artificial multi-enzyme systems.
Coenzymes and activators are often present in the cells, and...

Formation of reactive oxygen species (ROS) is a natural process during oxidative metabolism. ROS play an important role not only in pathological processes of human organism as usually presented but less attention is paid to their proper important role in c