Glycan Microarrays: Methods and Protocols

ISBN-13: 9781071621509 / Angielski / Miękka / 2021 / 253 str.

Michelle Kilcoyne; Jared Q. Gerlach

Opis

This volume encompasses short targeted reviews and methods featuring the construction and use of glycan microarray platforms. Chapters guider readers through glycan microarrays comprised of synthetic glycans, natural biomolecules, bacteria, neoglycoproteins,  various applications and progress of these platforms. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Glycan Microarrays: Methods and Protocols aims to highlight the interdisciplinary nature of the development and use of the glycan microarray platform.

Spis treści

Part I: Glycan microarray platforms

1. Neoglycoprotein and glycoprotein printing on a hydrogel functionalized microarray surface and incubation with labeled lectins

Jared Q. Gerlach, Marie Le Berre, and Michelle Kilcoyne

2. Evaluation of glycan-binding specificity by glycoconjugate microarray with an evanescent-field fluorescence detection system

Lalhaba Oinam and Hiroaki Tateno

3. Multiplex glycan bead array (MGBA) for high throughput and high content analyses of glycan binding proteins including natural anti-glycan antibodies

Sharad Purohit and Jin-Xiong She

4. Nanocube-based fluidic glycan array

Hung-Jen Wu, Akshi Singla, and Joshua D. Weatherston

 

Part II: Glycan microarray data extraction, processing and use for measurements

5. General strategies for glycan microarray data processing and analysis

J. Sebastian Temme and Jeffrey Gildersleeve

6. Calculating half maximal inhibitory concentration (IC₅₀) values from glycomics microarray data using GraphPad Prism

            Marie Le Berre, Jared Q. Gerlach, Iwona Dziembała, and Michelle Kilcoyne

 

Part III: Complex natural and synthetic glycoconjugate microarrays

7. Synthetic plant glycan microarrays as tools for plant biology

Colin Ruprecht and Fabian Pfrengle

8. Mucin purification and printing natural mucin microarrays

Marie Le Berre, Jared Q. Gerlach, Mary E. Gallagher, Lokesh Joshi, Stephen D. Carrington, and Michelle Kilcoyne

9. Bacterial microarrays for examining bacterial glycosignatures and recognition by host lectins

María Asunción Campanero-Rhodes, and Dolores Solís

10. Tissue glycome mapping: Lectin microarray-based differential glycomic analysis of formalin-fixed paraffin-embedded tissue sections

Chiaki Nagai-Okatani, Xia Zou, Atsushi Matsuda, Yoko Itakura, Masashi Toyoda, Yan Zhang, and Atsushi Kuno

 

Part IV: Applications for glycomics microarrays

11. Detection of autoantibodies using combinatorial glycolipid microarrays

Susan K. Halstead, Dawn Gourlay, and Hugh J. Willison

12. Glycan microarrays containing synthetic Streptococcus pneumoniae CPS fragments and their application to vaccine development

Paulina Kaplonek and Peter Seeberger

13. Lectin-based protein microarray for the glycan analysis of colorectal cancer biomarkers: the insulin-like growth factor system

Dragana Robajac, Martina Križáková, Miloš Šunderić, Goran Miljuš, Peter Gemeiner, Olgica Nedić, and Jaroslav Katrlík

14. Bacterial staining and profiling for glycan interactions on glycan microarrays for t-test calculation

Stephen Cunningham, Jared Q. Gerlach, Andrea Flannery, and Michelle Kilcoyne

15. Preparation and fluorescent labelling of cell-derived micelles and profiling on glycan microarrays

Marie Le Berre, Jared Q. Gerlach, and Michelle Kilcoyne