Structure and Function of Membrane Proteins

ISBN-13: 9781071613931 / Angielski / Twarda / 2021 / 358 str.

Ingeborg Schmidt-Krey; James C. Gumbart

Spis treści

1. Expression and Purification of Human Mitochondrial Intramembrane Protease PARL

            Elena Arutyunova, Laine Lysyk, Melissa Morrison, Cory Brooks, and M. Joanne Lemieux

 

2. Reconstitution of Detergent-Solubilized Membrane Proteins into Proteoliposomes and Nanodiscs for Functional and Structural Studies

            Kerry M. Strickland, Kasahun Neselu, Arshay J. Grant, Carolann L. Espy, Nael A. McCarty, and Ingeborg Schmidt-Krey

 

3. Biochemical Characterization of GPCR–G Protein Complex Formation

            Filip Pamula and Ching-Ju Tsai

 

4. Electrophysiological Approaches for the Study of Ion Channel Function

            Guiying Cui, Kirsten A. Cottrill, and Nael A. McCarty

 

5. Isothermal Titration Calorimetry of Membrane Proteins

            Han N. Vu, Alan J. Situ, and Tobias S. Ulmer

 

6. Atomic Force Microscopy Reveals Membrane Protein Activity at the Single Molecule Level

            Kanokporn Chattrakun, Katherine G. Schaefer, Lucas S. Chandler, Brendan P. Marsh, and Gavin M. King

 

7. Structure Determination of Membrane Proteins Using X-Ray Crystallography

            Evan Billings, Karl Lundquist, Claire Overly, Karthik Srinivasan, and Nicholas Noinaj

 

8. Studying Membrane Protein Structures by MicroED

            Michael W. Martynowycz and Tamir Gonen

 

9. Single-Particle Cryo-EM of Membrane Proteins

            Dovile Januliene and Arne Moeller

 

10. Helical Membrane Protein Crystallization in the New Era of Electron Cryo-Microscopy

            Mary D. Hernando, Joseph O. Primeau, and Howard S. Young

 

11. NMR Spectroscopic Studies of Ion Channels in Lipid Bilayers: Sample Preparation Strategies Exemplified by the Voltage Dependent Anion Channel

            Robert Silvers and Matthew Eddy

 

12. Preparation of a Deuterated Membrane Protein for Small-Angle Neutron Scattering

            Yuqi Wu, Kevin L. Weiss, and Raquel L. Lieberman

 

13. Preparing Membrane Proteins for Simulation Using CHARMM-GUI

            Yupeng Li, Jinchan Liu, and James C. Gumbart

 

14. Coarse-Grained Molecular Dynamics Simulations of Membrane Proteins: A Practical Guide

            William Glass, Jonathan W. Essex, Franca Fraternali, James Gebbie-Rayet, Irene Marzuoli, Marley L. Samways, Philip C. Biggin, and Syma Khalid

 

15. Continuous Constant pH Molecular Dynamics Simulations of Transmembrane Proteins

            Yandong Huang, Jack A. Henderson, and Jana Shen

 

16. Molecular Dynamics-Based Thermodynamic and Kinetic Characterization of Membrane Protein Conformational Transitions

            Dylan Ogden and Mahmoud Moradi

 

17. Concepts, Practices, and Interactive Tutorial for Allosteric Network Analysis of Molecular Dynamics Simulations

            Wesley M. Botello-Smith and Yun Lyna Luo

 

18. Large Scale Molecular Dynamics Simulations of Cellular Compartments

            Eric Wilson, John Vant, Jacob Layton, Ryan Boyd, Hyungro Lee, Matteo Turilli, Benjamín Hernández, Sean Wilkinson, Shantenu Jha, Chitrak Gupta, Daipayan Sarkar, and Abhishek Singharoy

Blurb

This book examines detailed experimental and computational approaches for the analysis of many aspects vital to the understanding of membrane protein structure and function. Readers will receive guidance on the selection and use of methods for over-expression and purification, tools to characterize membrane proteins within different phospholipid bilayers, direction on functional studies, and approaches to determine the structures of membrane proteins. Detailed experimental steps for specific membrane proteins with critical notes allow the protocols to be modified to different systems. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of practical information and implementation advice that leads to excellent, reproducible results. 

Authoritative and up-to-date, Structure and Function Studies of Membrane Proteins serves as an ideal guide for biologists, biochemists, and biophysicists striving to further understand these essential proteins and their many biological roles.