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Method development and validation of pharmaceutical dosage forms is a core area of quality control to assure the desired specifications in terms of strength, quality, purity, and identity of dosage form. The quality and safety of drugs can be maintained by estimating and monitoring the impurities effectively to assure that safe and effective drug formulations are available to consumers. High Performance Liquid Chromatography is the most versatile tool for the qualitative and quantitative analysis of drugs for analysis.

In the recent years there has been an exponential growth in the field of herbal medicine and these drugs are gaining popularity both in developing and developed countries because of their natural origin and less side effects In India, about 80% of the rural population uses medicinal herbs or indigenous systems of medicine. Polyphenolic compounds, flavonoids, terpenoids, saponins, polysaccharides and alkaloids are the major chemical moieties present in the plant species of various families and these major secondary metabolites tend to reverse or delay the diabetic complications.