Bioconjugate Techniques

ISBN-13: 9780123822390 / Angielski / Twarda / 2013 / 1200 str.

Greg Hermanson

Opis

Bioconjugate Techniques, 3rd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions, with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers.

• Offers a one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab
• Provides step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates
• Features full color illustrations
• Includes a more extensive introduction into the vast field of bioconjugation and one of the most thorough overviews of immobilization chemistry ever presented

Recenzje

"Most of the chapters address specific systems and reagents such as (strept) avidin-biotin, silane coupling, buckyballs & fullerenes, antibodies, liposome conjugates, and modified nucleotides-as well as a few chapters on observation techniques such as fluorescent probes and isotopic labeling. Also new since the last edition is a chapter addressing immobilization in chromatography." --ProtoView.com, February 2014

"This is an incomparable and essential guide for any scientist involved in conjugating biologically active molecules. The new edition does not disappoint with its liberal use of color (especially in reaction schemes), expanded pages, and newly added chapters. For those interested in bioconjugate chemistry, you may soon find that this is the only book on your research library shelf!" Rating: 5 Stars --Doody.com, February 2014

Spis treści

1. Introduction to Bioconjugation2. Functional Targets for Bioconjugation3. The Reactions of Bioconjugation4. Zero-Length Crosslinkers5. Homobifunctional Crosslinkers6. Heterobifunctional Crosslinkers7. Trifunctional Crosslinkers8. Dendrimers and Dendrons9. Cross-Bridges and Cleavable Reagent Systems10. Fluorescent Probes11. (Strept)avidin - Biotin Systems12. Isotopic Labeling Techniques13. Silane Coupling Agents14. Microparticles and Nanoparticles15. Immobilization of Ligands on Chromatography Supports16. Buckyballs, Fullerenes, and Carbon Nanotubes17. Chemoselective Ligation; Bioorthogonal Reagents18. PEGylation & Synthetic Polymer Modification19. Vaccine and Immunogen Conjugates20. Antibody Modification and Conjugation21. Liposome Conjugates and Derivatives22. Enzyme Modification and Conjugation23. Nucleic Acid and Oligonucleotide Modification and Conjugation24. Bioconjugation for the Study of Protein Interactions