Chromosome Analysis: Methods and Protocols

ISBN-13: 9781071624326 / Angielski / Twarda / 2022

Gotoh, Eisuke

Opis

This volume provides essential and fundamental protocols on manipulation chromosome. Chapters details methods on the preparation of mitotic chromosome, chromosome aberration, micronucleus (MN), comet assay, karyotyping, Fluorescent in situ hybridization (FISH), premature chromosome condensation (PCC), immunohistochemistry (IHC) staining, new generation sequencing technology and new chromosome concepts, such as epigenetic and its cause of cancer are presented. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Chromosome Analysis: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. 

Spis treści

1. A Basic and Simple Chromosome Preparation Protocol

Eisuke Gotoh

 

2. Metaphase chromosome preparation and classification of chromosomal aberrations

Takamitsu A Kato

 

3. Mitotic Index Analysis

Takamitsu A Kato and Jeremy S. Haskins

 

4. Preparation of Mitotic Cells for Fluorescence Microscopy

Yuji Tanno

 

5. Chemical induced Premature Chromosome Condensation protocol

Eisuke Gotoh

 

6. Apoptosis Detection Assays

Ilham Alshiraihi  and Takamitsu A Kato

 

7. Alkaline Comet Assay To Detect DNA Damage

Kade D. Walsh and Takamitsu A Kato

 

8. Analysis for Sister Chromatid Exchange

Takamitsu A Kato

 

9. Cytokinesis Blocked Micronuclei Aberration Analysis

Takamitsu A Kato

 

10. DNA Damage Foci on Metaphase Chromosomes

Takamitsu A Kato

 

11. FISH with Whole Chromosome Painting Probes

Takamitsu A Kato

 

12. Telomere aberration detection by PNA-FISH probe

Takamitsu A Kato

 

13. Nontraditional Method for Telomere Staining by PNA Probes

Takamitsu A Kato

 

14. Visualizing Active Replication Regions in S-phase Chromosomes

Eisuke Gotoh

 

15. Hi-C Analysis to Identify Genome-Wide Chromatin Structural Aberration in Cancer

Atsushi Okabe and Atsushi Kaneda

 

16. CUT&Tag using "stress-free" con A-conjugated Dynabeads®

Yasuhiro Fujiwara and Yuki Okada

 

17. Live-cell Synthetic Histone Acetylation by Chemical Catalyst

Shigehiro A. Kawashima and Motomu Kanai

 

18. Histone Modification Analysis of Low-Mappability Regions

Naoko Yoshizawa-Sugata and Hisao Masai1. A Basic and Simple Chromosome Preparation Protocol

Eisuke Gotoh

 

2. Metaphase chromosome preparation and classification of chromosomal aberrations

Takamitsu A Kato

 

3. Mitotic Index Analysis

Takamitsu A Kato and Jeremy S. Haskins

 

4. Preparation of Mitotic Cells for Fluorescence Microscopy

Yuji Tanno

 

5. Chemical induced Premature Chromosome Condensation protocol

Eisuke Gotoh

 

6. Apoptosis Detection Assays

Ilham Alshiraihi  and Takamitsu A Kato

 

7. Alkaline Comet Assay To Detect DNA Damage

Kade D. Walsh and Takamitsu A Kato

 

8. Analysis for Sister Chromatid Exchange

Takamitsu A Kato

 

9. Cytokinesis Blocked Micronuclei Aberration Analysis

Takamitsu A Kato

 

10. DNA Damage Foci on Metaphase Chromosomes

Takamitsu A Kato

 

11. FISH with Whole Chromosome Painting Probes

Takamitsu A Kato

 

12. Telomere aberration detection by PNA-FISH probe

Takamitsu A Kato

 

13. Nontraditional Method for Telomere Staining by PNA Probes

Takamitsu A Kato

 

14. Visualizing Active Replication Regions in S-phase Chromosomes

Eisuke Gotoh

 

15. Hi-C Analysis to Identify Genome-Wide Chromatin Structural Aberration in Cancer

Atsushi Okabe and Atsushi Kaneda

 

16. CUT&Tag using "stress-free" con A-conjugated Dynabeads®

Yasuhiro Fujiwara and Yuki Okada

 

17. Live-cell Synthetic Histone Acetylation by Chemical Catalyst

Shigehiro A. Kawashima and Motomu Kanai

 

18. Histone Modification Analysis of Low-Mappability Regions

Naoko Yoshizawa-Sugata and Hisao Masai