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In spite of tremendous scientific progress over the past years, cell biologists do not yet understand the fundamental processes that determine the life cy cle of a cell. Such are: cell movement and cell spreading, cell division, cell communication, cell signaling, cell regeneration and cell death. Biochemistry has enabled us to recognize and to isolate an overwhelming number of new proteins. In vitro assays and the reinjection of proteins into cells and tissues have provided insights into molecular functions and cellular mechanisms. The renaissance of the genetic approach by applying...

The standard protocols for the purification of all known cytoskeleton proteins are presented in this manual. Proteins are listed alphabetically and each protocol follows a common format. Thus, the manual provides a quick and easy reference to all relevant procedures for cytoskeleton protein purification.
The isolation procedure for each protein is shown in a clear flowchart, while the source of the protein, equipment and material needed, a list of suppliers, standard references, accession No. of sequences as well as further relevant facts and practical tips are given on a separate page.