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In Ecuador, as in other Latin American countries, bovine tuberculosis (BTB) is a major concern in the dairy cattle industry, with economic and public health importance. The increase of peri-urban dairy production associated with the absence of BTB control program in Ecuador are major risk factors in the context of tuberculosis. Furthermore, official data related to the disease are scarce and there is no national veterinary reference laboratory for mycobacteria in Ecuador. The present work was undertaken in this context. The main objectives were to provide data on the epidemiologic situation...