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The ability to control nonspecific protein adsorption at interfaces is a key issue in the design of biosensors, assays, chromatographic separations and implant materials. The research presented in this manuscript focuses on the effect of surface composition on the properties of adsorbed protein layers and the probing of protein-protein interactions both on adsorbed surfaces and in solution. Monolayers of alkanethiols on gold formed from solution self-assembly and microfluidic patterning using poly(dimethyl) siloxane (PDMS) served as platform for protein adsorption. We are employed surface...