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This fully updated book explores cerebrospinal fluid (CSF) molecular profiles in normal and pathological states, with a focus on mass spectrometry and different protein methods currently used in neurobiological disciplines. The book includes CSF sample preparation methods, shotgun and targeted proteomic workflows, as well as methods to characterize specific posttranslational modifications to achieve finer molecular resolution in proteomic studies of CSF. Additional chapters delve into experimental -omics strategies applied to the CSF lipidome and metabolome, as well as bioinformatic pipelines...