Quantitative Characterization of Ligand Binding

ISBN-13: 9780471059585 / Angielski / Miękka / 1995 / 176 str.

Donlad J. Winzor; Donald J. Winzor; William H. Sawyer

Opis

The book fills the void between rigorous theoretical discussions of ligand binding for physical chemists (often beyond the needs of bench experimentalists in the life sciences) and compilations of experimental recipes that offer scant regard for the appropriateness of the protocol for the particular ligand interaction under investigation.

Spis treści

Introduction: The Analysis of Simple Binding Responses.

Experimental Methods Based on Phase Separation.

Spectral Studies of Ligand Binding.

Other Direct Procedures for Characterizing Ligand Binding.

Binding Constants from Competitive Binding Assays.

Sigmoidal Binding Curves: Analysis and Interpretation.

Complicating Factors in the Analysis of Binding Curves.

Studies of DNA–Ligand Interactions.

Concluding Remarks.

Glossary of Symbols.

References.

Index.