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In the ten years since the first publication on PCR (Saiki et al. , 1985), this in vitro method of nucleic acid replication and modification has grown to rival in popularity traditional microbiological, genetical und technical procedures for cloning, sequencing, gene detecting and related procedures. To date the PCR literature has emphasized six main areas of application: genetic mapping, detection of mutations, genetic polymorphism, transcriptional splicing and regulation, molecular virology and quantitative procedures. The overwhelming focus of quantification of DNA or RNA by PCR has been...

The polymerase chain reaction (PCR) method of nucleic acid replication and modification now rivals traditional cloning, sequencing, and gene detecting procedures. Thirteen papers from the September 1996 meeting provide practical accounts of the authors' particular areas of PCR technology, and discus