Recombinant Protein Production in Yeast

ISBN-13: 9781493990238 / Angielski / Twarda / 2019 / 431 str.

Brigitte Gasser; Diethard Mattanovich

Spis treści

1. Established and Upcoming Yeast Expression Systems

 Burcu Gündüz Ergün, Damla Hüccetoğulları, Sibel Öztürk, Eda Çelik, and Pınar Çalık

 

2.Detection and Elimination of Cellular Bottlenecks in Protein Producing Yeasts

Richard J. Zahrl, Brigitte Gasser, Diethard Mattanovich, and Pau Ferrer

 

3.Pichia pastoris Promoters

Aysun Özçelik, Semiramis Yılmaz, and Mehmet Inan

 

4. Selection of the Optimal Yeast Host for the Synthesis Recombinant Enzymes

Felix Bischoff, Martin Giersberg, Falko Matthes, Tobias Schwalenberg, Sebastian Worch, and Gotthard Kunze

 

5. Screening and Selection of Production Strains: Secretory Protein Expression and Analysis in Hansenula polymorpha

Su Jin Yoo, Hye Yun Moon, and Hyun Ah Kang

 

6. Selection of Heterologous Protein Producing Strains in Yarrowia lipolytica

 Paul Soudier, Macarena Larroude, Ewelina Celinska, Tristan Rossignol, and Jean-Marc Nicaud

 

7. High-throughput Screening and Selection of Pichia pastoris Strains

Roland Weis

 

8. Analyzing Recombinant Protein Production in Pichia pastoris with Targeted Proteomics Roslyn M. Bill, Annegret Ulke-Lemée, Stephanie P. Cartwright, Rena Far, Jay Kim, and Justin A. MacDonald

 

9. CRISPR/Cas9-mediated Homology Directed Genome Editing in Pichia pastori

Thomas Gassler, Lina Heistinger, Diethard Mattanovich, Brigitte Gasser,  and Roland Prielhofer

 

10. Tailoring N-glycan Biosynthesis for Production of Therapeutic Proteins in Saccharomyces cerevisiae

Mari A. Piirainen, Heidi Salminen and Alexander D. Frey

 

11. Improving the Production of Cofactor Containing Proteins: Production of Human Hemoglobin in Yeast

Olena P. Ishchuk, José L. Martínez, and  Dina Petranovic

 

12. Membrane Protein Production in Yeast: Modification of Yeast Membranes for Human Membrane Protein Production

Anita Emmerstorfer-Augustin, Tamara Wriessnegger, Melanie Hirz, Guenther Zellnig, and  Harald Pichler

 

13. Yeast Surface Display and Cell Sorting of Antigen-binding Fc Fragments

Flávio Sádio, Gerhard Stadlmayr, Katharina Stadlbauer, Florian Rüker, and Gordana Wozniak-Knopp

 

14. Guidelines for Small-scale Production and Purification of Hepatitis B surface Antigen Virus-like Particles from Recombinant Pichia pastoris

Maria Zahid and Ursula Rinas

 

15. Efficient Development of a Mixed Feed Process for Pichia pastoris

David Johannes Wurm and Oliver Spadiut

 

16. Primary Recovery of Yeast Culture Supernatant for Recombinant Protein Purification

Michael M. Maurer and Harald Schillinger

 

17. Purification of Recombinant Glyco-proteins from Pichia pastoris Culture Supernatants

David Johannes Wurm and Oliver Spadiut

 

18. LC-MS analysis of (glyco)-proteins of Pichia pastoris

Clemens Grünwald-Gruber and Friedrich Altmann

 

19. Assessing the Quality of Recombinant Products Made in Yeast

Karola Vorauer-Uhl and Gabriele Lhota

 

20. Quantification of Recombinant Products In Yeast

Karola Vorauer-Uhl and Gabriele Lhota

Blurb

This volume provides an overview of the main yeast production platforms currently used and future yeast cell factories for recombinant protein production. Chapters detail approaches of genetic and metabolic engineering, co-factor containing proteins and virus-like particles, glycoproteins, and post-translational modifications of proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Recombinant Protein Production in Yeast: Methods and Protocols aims to provide state of the art background and methods for protein producing yeast platforms, as well as case studies for special applications.