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To evaluate the anti-cancer activity of Limonia acidissima Linn (Rutaceae) which has long been used traditionally for various infectious and malignant diseases. The fruit extract of Limonia acidissima Linn was obtained by macerating 3 times with methanol and then concentrating it at reduced pressure. Bioassay of the extract was carried out to assess the anticancer activity. Breast cancer cell lines, SKBR3 and MDA MB435, were used for in-vitro cell proliferation, cell viability assay, and cell cycle analysis of the extract.Results: Bioassay of the extract of Limonia acidissima Linn. showed...