Developmental Competence of In-vitro Matured Goat Oocytes : Studies on developmental competence of in-vitro matured goat oocyetes in a medium with different supplementation

ISBN-13: 9783330025752 / Angielski / Miękka / 2016 / 124 str.

Kaushlendra Pratap Singh; Atul Saxena S.D. Kharche

Opis

In-vitro embryo production provide an excellent source of embryos for carrying basic research on developmental physiology, farm animal breeding and for commercial application of the emerging biotechniques like embryo sexing, sperm injection, nuclear transfer, cloning and transgenic. The technique of in-vitro maturation, fertilization and culture of embryo essentially involve collection of oocytes from slaughter house ovaries or from living animals through transvaginal aspiration, in-vitro maturation of selected oocytes in media containing serum and hormones, in-vitro fertilization either with frozen thawed semen or freshly collected in-vitro capacitated spermatozoa and in-vitro culture of embryos in media containing oviduct or cumulus cell up to the morulae or blastocyst stage, at which stage they can be either transferred to synchronized recipients or frozen and stored. The present study is aimed to evaluate the effects of different supplementation in the basic media on in-vitro maturation and cleavage rate and to study the developmental competence of in-vitro fertilized goat oocytes on granulosa cell monolayers and oviductal epethelial cells co-culture.

O autorze

Dr. Kaushlendra Pratap Singh born on 20th may, 1976 in Lucknow, is working as Veterinary Medical Officer in Dept. of Animal Husbandry, Uttar Pradesh, India. He obtained his B.V.Sc.& A.H. from G.B.P.U.A.T,Pantnagar in 2003 and M.V.Sc. from Department of Obstetrics and Gynaecology DUVASU, Mathura in 2005. He has published 149 publication in his name.