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In the present book, tissue culture studies on two medicinally important plant species- Bacopa monnieri and Tylophora indica is presented which was carried out as a work for M.Sc. in 2010. Tissue culture methods are now routinely adopted for propagating medicinal plants as they are being depleted from wild. In vitro propagation includes five different stages and stage-III is rooting of microshoots in static or liquid media. It is an important stage as after that the in vitro plants are transferred to the field. Rooting can also be induced by using natural planting substrates which is...