Wyniki wyszukiwania:

Penicillin acylase (PA) is an industrial enzyme used for hydrolysis of penicillin to -lactam nucleus 6-aminopenicillanic acid (6-APA) using for manufacturing semi-synthetic penicillins. Microbial enzyme transformation processes are generally preferred over the conventional chemical conversion process. Bacterial strains such as Escherichia coli are widely used for industrial production of 6-APA. The study provides a comprehensive resource for i) screening/characterization of PA producing E. coli strains from different habitats, ii) optimization of conditions for PA production and iii)...

Bacterial enzymes such as penicillin acylases are used for biosynthesis of betalactam antibiotics. The present study highlights the importance of manipulation of microbiological media and methods for isolation and characterization of bacterial strains having potential for penicillin acylases. Simple approach has been adopted for developing mutants with enhanced production of penicillin acylase. Bacterial strains collected from different ecological niches have unique properties and manifests diverse levels of enzyme activity. The high diversity in the natural population demonstrates that broad...