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This book consists of different DNA manipulation methods (BioBrick, SLIC, CPEC, Golden Gate, GoldenBraid, MoClo, USER etc.) assembly techniques (MAGE and CAGE), genetic regulations (modifying ribosomal parts, carboxysomal subunits) via modifying numerous metabolic pathways (Mevalonate pathway, Glucaric acid pathway etc.) leading to recreation of cells or artificial cellular structures. It have immense hope for future of mankind by different application in biofuel productions, cancer therapy, bioremediation, crop engineering, biodegradation etc.

The occurrence of fluoride at elevated concentrations in different environmental compartments in almost all parts of the world poses a real threat to life on the earth. The hazardous effect of fluoride includes damage of human biological systems, even at molecular level and leads to serious health disorder, the fluorosis. The resulting manifestations of fluorosis are the mottling of teeth, softening of bones, ossification of tendons and ligaments and also neurological damage in severe condition. The present work highlights the occurrence and distribution of fluoride in ground water in...