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From probe design to applications in clinical settings, this book provides a diverse set of instructive examples, guided by experts in the field who offer easy-to-follow experimentals. The book first offers an introduction to the basic principles of fluorescence and then describes applications of fluorogenic probes in real-time PCR, which currently is the gold standard for quantitative DNA and RNA analysis.

Coverage extends the potential of realtime as well as advocates simplifications of the probe technologies. It also presents a new simplified molecular beacon design,...

From probe design to applications in clinical settings, this book provides a diverse set of instructive examples, guided by experts in the field who offer easy-to-follow experimentals. The book first offers an introduction to the basic principles of fluorescence and then describes applications of fluorogenic probes in real-time PCR, which currently is the gold standard for quantitative DNA and RNA analysis.

Coverage extends the potential of realtime as well as advocates simplifications of the probe technologies. It also presents a new simplified molecular beacon design,...

Der vorliegende Band 2 der Reihe Empirische Didaktik / Fachmethodik liefert Beiträge zu Unterrichtsmethoden für den Mathematikunterricht. Der Begriff Unterrichtsmethode hat eine besondere Bedeutung für den vorliegenden Band. Er wird verstanden als ein klar umrissener, begrifflich herauslösbarer, selbst-ständiger, wenn auch integrierter Bestandteil des Unterrichts . Unterrichtsmethoden in diesem Sinne sind etwa problemorientierter Unterricht, entdeckendes Lernen, Computersimulation, Frontalunterricht, Modellmethode. Insgesamt werden für 20 Unterrichtsmethoden explizit die...