Myeloid-Derived Suppressor Cells

ISBN-13: 9781071610596 / Angielski / Twarda / 2020 / 220 str.

Sven Brandau; Anca Dorhoi

Spis treści

Preface…
Table of Contents...
Contributing Authors...

1. Immunophenotyping of Circulating Myeloid-Derived Suppressor Cells (MDSC) in the Peripheral Blood of Cancer Patients
Kirsten Bruderek, Ronja Schirrmann, and Sven Brandau

2. Phenotyping of Myeloid-Derived Suppressor Cells in Nonhuman Primates
Ang Lin and Karin Loré 

3. Isolation and Phenotyping of Splenic Myeloid-Derived Suppressor Cells in Murine Cancer Models
Emilio Sanseviero, Rina Kim, and Dmitry I. Gabrilovich

4. Phenotypical Characterization and Isolation of Tumor-Derived Mouse Myeloid-Derived Suppressor Cells
Roza Maria Barouni, Chiara Musiu, Vincenzo Bronte, Stefano Ugel, and Stefania Canè

5. Isolation of Human Circulating Myeloid-Derived Suppressor Cells and Analysis of Their Immunosuppressive Activity
Kirsten Bruderek, Ronja Schirrmann, and Sven Brandau

6. High Dimensional Phenotyping of Human Myeloid-Derived Suppressor Cells/Tumor-Associated Macrophages in Tissue by Mass Cytometry
Juliette Ferrant, Simon Le Gallou, Guillaume Manson, Steve Genebrier, Frederic Mourcin, Karin Tarte, and Mikael Roussel

7. Depletion and Maturation of Myeloid-Derived Suppressor Cells in Murine Cancer Models
Christopher Groth, Rebekka Weber, Jochen Utikal, and Viktor Umansky

8. In Vitro Generation of Human Neutrophilic Myeloid-Derived Suppressor Cells 
Anurag Singh and Nikolaus Rieber

9. Measuring Suppressive Activity and Autophagy in Myeloid-Derived Suppressor Cells
Antonis Stylianos Papaioannou, Athina Boumpas, Miranta Papadopoulou, Aikaterini Hatzioannou, Themis Alissafi, and Panayotis Verginis 

10. In Vitro Generation of Murine Myeloid-Derived Suppressor Cells, Analysis of Markers, Developmental Commitment, and Function
Ina Eckert, Eliana Ribechini, and Manfred B. Lutz

11. Analysis of Antimicrobial Activity of Monocytic Myeloid-Derived Suppressor Cells in Infection with Mycobacterium tuberculosis and Human Immunodeficiency Virus
Ankita Garg

12. Isolation and Functional Characterization of Myeloid-Derived Suppressor Cells in Infections Under High Containment
Leigh A. Kotze, Vinzeigh N. Leukes, and Nelita du Plessis

13. High-Dimensional Analysis of Circulating and Tissue-Derived Myeloid-Derived Suppressor Cells from Patients with Glioblastoma
Tyler J. Alban, Defne Bayik, Alvaro G. Alvarado, Harley I. Kornblum, and Justin D. Lathia

14. Single Cell Transcriptome Analysis Workflow for Splenic Myeloid-Derived Suppressor Cells from Murine Breast Cancer Models
Hamad Alshetaiwi, Nicholas Pervolarakis, Quy H. Nguyen, and Kai Kessenbrock

15. Intravital 2-Photon Microscopy of Diverse Cell Types in the Murine Tibia
Anja Hasenberg, Lucas Otto, and Matthias Gunzer

16. Isolation of Bovine Neutrophils by Fluorescence- and Magnetic-Activated Cell Sorting
Marion Rambault, Rachana Borkute, Emilie Doz-Deblauwe, Yves Le-Vern, Nathalie Winter, Anca Dorhoi, and Aude Remot

Subject Index List…

Blurb

This volume explores various experimental approaches that can be used to investigate myeloid-derived suppressor cells (MDSC) and can be useful to understand the characterization of these cells. The chapters in this book cover topics such as the study of MDSC in human patient samples; employing flow cytometry or magnetic enrichment; immunophenotyping using multi-parameter flow cytometry and mass cytometry; functional characterization of MDSC by biochemical, immunological, and microscopy approaches; MDSC characterization through the use of high-resolution genomics; and novel methods of high-resolution visualization of living tissue and the purification of granulocytes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Myeloid-Derived Suppressor Cells: Methods and Protocols is a valuable resource for novices in this field to help them start their research on MDSC, and experienced investigators looking to establish new protocols in their lab. This book is great for anyone working in the field of biology, medicine, and veterinary medicine.