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The main objective of this study was to determine and understand how environmental factors influence growth and the regulation of AFB1 production, and to gain knowledge of the correlation of ecophysiological conditions with toxin gene expression of the aflatoxin genes in relation to phenotypic production. In this study the following studies have been carried out: Effect of combinations of water activity x temperature interactions on growth, and sporulation, and partitioning of AFB1 into spores, mycelium and medium using A. flavus NRRL 3357. Use of a microarray and real-timePCR to examine the...

The school bus routing problem (SBRP) is a central issue in transportation planning and optimization systems. SBRP seeks to plan an efficient schedule for a fleet of school buses where each bus picks up students from various bus stops and delivers them to their designated schools while satisfying various constraints such as the bus capacity. Due to its complexity, many heuristics have been proposed to solve this combinatorial problem in an effective way. In this book, geographic information systems (GIS)-based decision-making framework that combines GIS, clustering techniques, network cutting...