Peptide Synthesis and Applications

ISBN-13: 9781627035439 / Angielski / Twarda / 2013 / 253 str.

Knud J. Jensen; A. Pernille Tofteng; Soren L. Pedersen

Opis

Peptides are used ubiquitously for studies in biology, biochemistry, chemical biology, peptide based medicinal chemistry, and many other areas of research. There is a number of marketed peptide drugs, and the prospects for the development of new peptide drugs are very encouraging. The second edition of "Peptide Synthesis and Applications" expands upon the previous editions with current, detailed methodologies for peptide synthesis. With new chapters on laboratory protocols for both the specialist and the non-specialist. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, "Peptide Synthesis and Application, Second Edition "seeks to aid scientists in understanding different approaches to the synthesis of peptides by using a broad range of methods and strategies.

Spis treści

1. Solid-Phase Peptide Synthesis: An Introduction

Knud J. Jensen

 

2. Linkers, Resins and General Procedures for Solid-phase Peptide Synthesis

A. Pernille Tofteng and Knud J. Jensen

 

3. Peptide Release, Side-chain Deprotection, Work-up, and Isolation

Søren L. Pedersen and Knud J. Jensen

 

4. Synthesis of Peptides using Tert-butyloxycarbonyl (Boc) as the α-amino Protection Group

Søren W. Pedersen, Christopher J. Armishaw and Kristian Strømgaard

 

5. Sequential Formation of Regioselective Disulfide Bonds in Synthetic Peptides with Multiple Disulfide Bonds

Fazel Shabanpoor, Mohammed Akhter Hossain, Feng Lin, and John D. Wade

 

6. Synthesis of Cyclic Disulfide-Rich Peptides

Muharrem Akcan and David J. Craik

 

7. Preparation of C-terminally Modified Chemokines by Expressed Protein Ligation

Lars Baumann, Max Steinhagen , Annette G. Beck-Sickinger

 

8. Synthesis of C-terminal Peptide Thioesters using Fmoc Based Solid-phase Peptide Chemistry

A. Pernille Tofteng and Knud J. Jensen

 

9. Backbone Amide Linker Strategy –Protocols for the Synthesis of C‑terminal Peptide Aldehydes

A. Pernille Tofteng and Knud J. Jensen

 

10. Synthesis of N-methylated Peptides: On-resin Methylation and Microwave-Assisted Couplings

Renée Roodbeen and Knud J. Jensen

 

11. Synthesis of Antimicrobial Peptoids

Paul R. Hansen and Jens K. Munk

 

12. Synthesis of Lipidated Peptides

Federica Rosi and Gemma Triola

 

13. Solid-Phase Synthesis of Phosphopeptides

Kim B. Højlys-Larsen and Knud J. Jensen

 

14. Synthesis of O-Glycopeptides and Construction of Glycopeptide Microarrays

Ola Blixt and Emiliano Cló

 

15. Instruments for Automated Peptide Synthesis

Søren L. Pedersen and Knud J. Jense

 

16. Microwave-Assisted Solid-Phase Peptide Synthesis using the Biotage Syro Wave™

Leila Malik and Knud J. Jensen

 

17. Microwave-Assisted Solid-Phase Peptide Synthesis Based on the Fmoc Protecting Group Strategy (CEM)

Grace S. Vanier