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Out of 1500 nasopharyngeal swab samples collected from patients who were admitted to different maternity clinics in Dhaka city, 111 optochin resistant strains were isolated. As a confirmatory test of Streptococcus pneumoniae, i.e., Bile solubility was checked. A total of 37 optochin resistant bile soluble isolates were obtained, designated as optochin resistant bile soluble S. pneumoniae as other - hemolytic streptococci are not bile soluble. For further identification, bile soluble isolates were subjected to PCR of the lytA gene specific for S. pneumonia an 32 (87%) isolates were...