enzyme kinetics: rapid-equilibrium applications of mathematica

ISBN-13: 9780470639320 / Angielski / Twarda / 2011 / 456 str.

Robert A. Alberty;¬Abar;¬Abar

Opis

Rapid-Equilibrium Enzyme Kinetics helps readers emphasize the estimation of kinetic parameters with the minimum number of velocity measurements, thereby reducing the amount of laboratory work necessary, and allowing more time for the consideration of complicated mechanisms. The book systematically progresses through six levels of understanding the enzyme-catalyzed reaction, and includes a CD-ROM so that the reader may use the programs in the book to input their own experimental data.

Spis treści

Preface.

Chapter 1 Biochemical Thermodynamics.

Chapter 2 A = P.

Chapter 3 Ordered A + B –> Products.

Chapter 4 Random A + B –> Products.

Chapter 5 A + B = P + Q.

Chapter 6 A + B + C –> Products.

Chapter 7 Ordered O + mR –> Products.

Chapter 8 Random O + mR –> Products.

Chapter 9 Inhibition and Activation of A –> Products.

Chapter 10 Modification of A –> Products.

Chapter 11 Inhibition, Activation, and Modification of A + B –> Products.

Chapter 12 Systems of Enzyme–Catalyzed Reactions.

References.

Index.

O autorze

Robert A. Alberty received a BS in 1943 from the University of Nebraska and a PhD from the University of Wisconsin in 1947. He is the author of Thermodynamics of Biochemical Reactions in 2003 and Biochemical Thermodynamics in 2006, both from Wiley. In 2004, he coauthored Physical Chemistry, Fourth Edition with Robert J. Silbey and Moungi G. Bawendi.

Blurb

Navigate the complexities of enzyme kinetics with Mathematica

Quantitative calculations on the rates of enzyme–catalyzed reactions can help identify mechanisms of catalysis and calculate the speed of catalyzed reactions under various conditions. This book shows how to use mathematical applications such as Mathematica to conduct these calculations more easily and for more complicated mechanisms.

• Systematically describes the kinetics of an enzyme–catalyzed reaction using six steps, or six levels of understanding

• Provides the rate equations for a variety of types of enzyme–catalyzed reactions under various temperature, pH, and ionic strengths

• Requires a minimal number of velocity measurements in order to determine kinetic parameters for enzymatic reactions

• Shows step–by–step instructions for these calculations plus worked–out examples

• Provides a CD–ROM with the entire text of the book as well as programs you can use to estimate kinetic parameters from your own experimental data