Yeast Surface Display

ISBN-13: 9781071622841 / Angielski / Twarda / 2022

Opis

This detailed volume explores a wide variety of applications of yeast surface display, an extensively used protein engineering technology. Beginning with detailed protocols for the construction and efficient selection/screening of yeast surface display libraries, as well as for the analysis of individual yeast-displayed protein variants, the book continues with protocols describing the selection of yeast surface display libraries for binding to mammalian cells or to extracellular matrix as well as protocols for a broad spectrum of specialized yeast surface display applications, demonstrating the versatility of this display platform. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible methodologies, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Yeast Surface Display serves as a comprehensive resource that enables the implementation of this powerful and versatile technique in virtually any molecular biology laboratory, even in the absence of any prior yeast surface display experience.

Spis treści

Part I: Introduction to Yeast Surface Display and Its Applications

 

1. Yeast Surface Display:  New Opportunities for a Time-Tested Protein Engineering System

            Maryam Raeeszadeh-Sarmazdeh and Eric T. Boder

 

Part II: Construction and Selection of Yeast Surface Display Libraries and Analysis of Isolated Variants

 

2. Yeast Surface Display for Protein Engineering: Library Generation, Screening, and Affinity Maturation

            Byong H. Kang, Brianna M. Lax, and K. Dane Wittrup

 

3. Site-Wise Diversification of Combinatorial Libraries Using Insights from Structure-Guided Stability Calculations

            Benedikt Dolgikh and Daniel Woldring

 

4. Ancestral Sequence Reconstruction and Alternate Amino Acid States Guide Protein Library Design for Directed Evolution

            James VanAntwerp, Patrick Finneran, Benedikt Dolgikh, and Daniel Woldring

 

5. Machine Learning-Driven Protein Library Design: A Path Toward Smarter Libraries

            Mehrsa Mardikoraem and Daniel Woldring

 

6. Kinetic Competition Screening of Yeast-Displayed Libraries for the Generation of High Affinity Binders

            Nicole J. Yang

 

7. Engineering Proteins by Combining Deep Mutational Scanning and Yeast Display

            Preeti Sharma, Erik Procko, and David M. Kranz

 

8. Engineering Binders with Exceptional Selectivity

            Kai Wen Teng, Akiko Koide, and Shohei Koide

 

9. Affinity and Stability Analysis of Yeast Displayed Proteins

            Charlotte U. Zajc, Magdalena Teufl, and Michael W. Traxlmayr

 

Part III: Selection of Yeast Surface Display Libraries for Binding to Mammalian Cells or Extracellular Matrix

 

10. Antibody Library Screening Using Yeast Biopanning and Fluorescence-Activated Cell Sorting

            Stefania C. Carrara, Jan P. Bogen, Julius Grzeschik, Björn Hock, and Harald Kolmar

 

11. A Hybrid Adherent/Suspension Cell-Based Selection Strategy for Discovery of Antibodies Targeting Membrane Proteins

            Patrick J. Krohl and Jamie B. Spangler

 

12. Ligand Selection by Combination of Recombinant and Cell Panning Selection Techniques

            Rojhae A. Panton and Lawrence A. Stern

 

13. Identification of Brain ECM Binding Variable Lymphocyte Receptors Using Yeast Surface Display

            Benjamin J. Umlauf, John S. Kuo, and Eric V. Shusta

 

Part IV: Specialized Yeast Surface Display Applications

 

14. Guidelines, Strategies, and Principles for the Directed Evolution of Cross-Reactive Antibodies Using Yeast Surface Display Technology

            Sara Linciano, Ee Lin Wong, Ylenia Mazzocato, Monica Chinellato, Tiziano Scaravetti, Alberto Caregnato, Veronica Cacco, Zhanna Romanyuk, and Alessandro Angelini

 

15. Yeast Display for the Identification of Peptide-MHC Ligands of Immune Receptors

            Brooke D. Huisman, Beth E. Grace, Patrick V. Holec, and Michael E. Birnbaum

 

16. Yeast Display Guided Selection of pH Dependent Binders

            Jenna N. Meanor, Albert J. Keung, Balaji M. Rao, and Nimish Gera

 

17. Yeast Mating as a Tool for Highly Effective Discovery and Engineering of Antibodies via Display Methodologies

            Du-San Baek, Seong-Wook Park, Cynthia Adams, Dimiter S. Dimitrov, and Yong-Sung Kim

 

18. Humanization of Chicken-Derived Antibodies by Yeast Surface Display

            Jan P. Bogen, Adrian Elter, Julius Grzeschik, Björn Hock, and Harald Kolmar

 

19. Engineering Tissue Inhibitors of Metalloproteinases Using Yeast Surface Display

            Mari R. Toumaian and Maryam Raeeszadeh-Sarmazdeh

 

20. Discovery of Cyclic Peptide Binders from Chemically Constrained Yeast Display Libraries

            Kaitlyn Bacon, Stefano Menegatti, and Balaji M. Rao

 

21. Generation of Thermally Stable Affinity Pairs for Sensitive, Specific Immunoassays

            Elliot Corless, Yining Hao, Huan Jia, Patthara Kongsuphol, Dousabel Tay, Say Yong Ng, and Hadley D. Sikes

 

22. Isolating Anti-Amyloid Antibodies from Yeast-Displayed Libraries

            Alec A. Desai, Jennifer M. Zupancic, Matthew D. Smith, and Peter M. Tessier

 

23. Engineering Proteins Containing Noncanonical Amino Acids on the Yeast Surface

            Rebecca Hershman, Arlinda Rezhdo, Jessica Stieglitz, and James A. Van Deventer

 

24. Construction of Yeast Display Libraries for Selection of Antigen-Binding Variants of Large Extracellular Loop of CD81, a Major Surface Marker Protein of Extracellular Vesicles

            Stefan Vogt, Gerhard Stadlmayr, Katharina Stadlbauer, Florian Stracke, Madhusudhan Reddy Bobbili, Johannes Grillari, Florian Rüker, and Gordana Wozniak-Knopp

 

25. Isolating and Engineering Fluorescence-Activating Proteins Using Yeast Surface Display

            Lina El Hajji, Hela Benaissa, and Arnaud Gautier

 

26. Simultaneous Display of Multiple Kinds of Enzymes on the Yeast Cell Surface for Multistep Reactions

            Kouichi Kuroda and Mitsuyoshi Ueda